Data from: Identification and functional characterization of immunity-suppressing, candidate effector proteins in the parasitic weed Phelipanche aegyptiaca
Principal Investigator(s): View help for Principal Investigator(s) US Department of Agriculture; National Agricultural Library
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Project Citation:
US Department of Agriculture, and National Agricultural Library. Data from: Identification and functional characterization of immunity-suppressing, candidate effector proteins in the parasitic weed Phelipanche aegyptiaca. Ann Arbor, MI: Inter-university Consortium for Political and Social Research [distributor], 2026-06-27. https://doi.org/10.3886/E250363V1
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Data from: Identification and functional characterization of immunity-suppressing, candidate effector proteins in the parasitic weed Phelipanche aegyptiaca
Summary:
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All source data from the referenced paper (Figures 1b and Table 1). 22 Excel files of data from each experimental block of the reactive oxygen species assay, 1 Excel file of the combined data from the bacterial growth enhancement assay, 1 Excel file of the RT-qPCR data.
Parasitic plants are the primary biotic constraint in many crop production systems. The most agriculturally devastating parasitic plants, including witchweeds (Striga spp.) and broomrapes (Phelipanche and Orobanche spp.), are in the Orobanchaceae family. Phelipanche aegyptiaca is an obligate holoparasite that lacks the capacity for photosynthesis and therefore relies on host parasitization for acquisition of all nutrients. P. aegyptiaca is a broad host range pathogen with the ability to parasitize diverse dicot hosts through attachment and development of a feeding structure known as a haustorium. The mechanisms P. aegyptiaca and other parasitic plants employ to avoid host plant immunity and form successful haustorial attachments are unknown. Here, we demonstrate that P. aegyptiaca actively suppresses salicylic acid-mediated immunity of the host plant Arabidopsis thaliana. We hypothesized that parasitic plants may deploy immunity-suppressing effector proteins through the haustorial interface to subvert host plant immune responses. We devised a pipeline to select and clone 27 candidate secreted effector proteins in P. aegyptiaca and tested these proteins for the potential to suppress known plant immunity pathways. Five candidate effectors suppressed flg22-elicited production of reactive oxygen species when transiently expressed in Nicotiana benthamiana. We propose that two of these candidate effectors function through interfering with pattern triggered immunity using molecular mimicry.
Resources in this dataset:
Resource Title: Data from Identification and functional characterization of immunity-suppressing, candidate effector proteins in the parasitic weed Phelipanche aegyptiaca. File Name: AllSourceDataPaegEffectorPaper.zipResource Description: All source data from the referenced paper.
Resource Title: Data from Identification and functional characterization of immunity-suppressing, candidate effector proteins in the parasitic weed Phelipanche aegyptiaca.
File Name: AllSourceDataPaegEffectorPaper.zip
Resource Description: All source data from the referenced paper.
Parasitic plants are the primary biotic constraint in many crop production systems. The most agriculturally devastating parasitic plants, including witchweeds (Striga spp.) and broomrapes (Phelipanche and Orobanche spp.), are in the Orobanchaceae family. Phelipanche aegyptiaca is an obligate holoparasite that lacks the capacity for photosynthesis and therefore relies on host parasitization for acquisition of all nutrients. P. aegyptiaca is a broad host range pathogen with the ability to parasitize diverse dicot hosts through attachment and development of a feeding structure known as a haustorium. The mechanisms P. aegyptiaca and other parasitic plants employ to avoid host plant immunity and form successful haustorial attachments are unknown. Here, we demonstrate that P. aegyptiaca actively suppresses salicylic acid-mediated immunity of the host plant Arabidopsis thaliana. We hypothesized that parasitic plants may deploy immunity-suppressing effector proteins through the haustorial interface to subvert host plant immune responses. We devised a pipeline to select and clone 27 candidate secreted effector proteins in P. aegyptiaca and tested these proteins for the potential to suppress known plant immunity pathways. Five candidate effectors suppressed flg22-elicited production of reactive oxygen species when transiently expressed in Nicotiana benthamiana. We propose that two of these candidate effectors function through interfering with pattern triggered immunity using molecular mimicry.
Resources in this dataset:
Resource Title: Data from Identification and functional characterization of immunity-suppressing, candidate effector proteins in the parasitic weed Phelipanche aegyptiaca. File Name: AllSourceDataPaegEffectorPaper.zipResource Description: All source data from the referenced paper.
Resource Title: Data from Identification and functional characterization of immunity-suppressing, candidate effector proteins in the parasitic weed Phelipanche aegyptiaca.
File Name: AllSourceDataPaegEffectorPaper.zip
Resource Description: All source data from the referenced paper.
Original Distribution URL:
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https://agdatacommons.nal.usda.gov/articles/dataset/Data_from_Identification_and_functional_characterization_of_immunity-suppressing_candidate_effector_proteins_in_the_parasitic_weed_Phelipanche_aegyptiaca/24664671
Scope of Project
Subject Terms:
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NP303;
data.gov;
ARS
Collection Notes:
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DOI: 10.15482/USDA.ADC/1503914
Citation: Clarke, Christopher R. (2019). Data from: Identification and functional characterization of immunity-suppressing, candidate effector proteins in the parasitic weed Phelipanche aegyptiaca. Ag Data Commons. https://doi.org/10.15482/USDA.ADC/1503914 (Downloaded 2026-06-26)
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