Data from: Identification of Single-Nucleotide Polymorphic Loci Associated with Biomass Yield under Water Deficit in Alfalfa (Medicago sativa L.) Using Genome-Wide Sequencing and Association Mapping
Principal Investigator(s): View help for Principal Investigator(s) US Department of Agriculture; National Agricultural Library
Version: View help for Version V1
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Project Citation:
US Department of Agriculture, and National Agricultural Library. Data from: Identification of Single-Nucleotide Polymorphic Loci Associated with Biomass Yield under Water Deficit in Alfalfa (Medicago sativa L.) Using Genome-Wide Sequencing and Association Mapping. Ann Arbor, MI: Inter-university Consortium for Political and Social Research [distributor], 2026-06-27. https://doi.org/10.3886/E250348V1
Project Description
Project Title:
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Data from: Identification of Single-Nucleotide Polymorphic Loci Associated with Biomass Yield under Water Deficit in Alfalfa (Medicago sativa L.) Using Genome-Wide Sequencing and Association Mapping
Summary:
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Alfalfa is a worldwide grown forage crop and is important due to its high biomass production and nutritional value. However, the production of alfalfa is challenged by adverse environmental factors such as drought and other stresses. Developing drought resistance alfalfa is an important breeding target for enhancing alfalfa productivity in arid and semi-arid regions. In the present study, we used genotyping-by-sequencing and genome-wide association to identify marker loci associated with biomass yield under drought in the field in a panel of diverse germplasm of alfalfa. A total of 28 markers at 22 genetic loci were associated with yield under water deficit, whereas only four markers associated with the same trait under well-watered condition. Comparisons of marker-trait associations between water deficit and well-watered conditions showed non-similarity except one. Most of the markers were identical across harvest periods within the treatment, although different levels of significance were found among the three harvests. The loci associated with biomass yield under water deficit located throughout all chromosomes in the alfalfa genome agreed with previous reports. Our results suggest that biomass yield under drought is a complex quantitative trait with polygenic inheritance and may involve a different mechanism compared to that of non-stress. BLAST searches of the flanking sequences of the associated loci against DNA databases revealed several stress-responsive genes linked to the drought resistance loci, including leucine-rich repeat receptor-like kinase, B3 DNA-binding domain protein, translation initiation factor IF2, and phospholipase-like protein. With further investigation, those markers closely linked to drought resistance can be used for MAS to accelerate the development of new alfalfa cultivars with improved resistance to drought and other abiotic stresses.
Resources in this dataset:
Resource Title: Alfalfa accessions used in GWAS for drought resistance. File Name: table 1.csvResource Description: Original file can be downloaded from https://www.frontiersin.org/articles/file/downloadfile/264653_supplementary-materials_tables_1_xlsx/octet-stream/Table 1.XLSX/1/264653
Resource Title: Alfalfa accessions used in GWAS for drought resistance.
File Name: table 1.csv
Resource Description: Original file can be downloaded from https://www.frontiersin.org/articles/file/downloadfile/264653_supplementary-materials_tables_1_xlsx/octet-stream/Table 1.XLSX/1/264653
Resources in this dataset:
Resource Title: Alfalfa accessions used in GWAS for drought resistance. File Name: table 1.csvResource Description: Original file can be downloaded from https://www.frontiersin.org/articles/file/downloadfile/264653_supplementary-materials_tables_1_xlsx/octet-stream/Table 1.XLSX/1/264653
Resource Title: Alfalfa accessions used in GWAS for drought resistance.
File Name: table 1.csv
Resource Description: Original file can be downloaded from https://www.frontiersin.org/articles/file/downloadfile/264653_supplementary-materials_tables_1_xlsx/octet-stream/Table 1.XLSX/1/264653
Original Distribution URL:
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https://agdatacommons.nal.usda.gov/articles/dataset/Data_from_Identification_of_Single-Nucleotide_Polymorphic_Loci_Associated_with_Biomass_Yield_under_Water_Deficit_in_Alfalfa_Medicago_sativa_L_Using_Genome-Wide_Sequencing_and_Association_Mapping/24662886
Scope of Project
Subject Terms:
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alfalfa;
forage crops;
biomass production;
nutritive value;
environmental factors;
drought;
drought tolerance;
breeding;
semiarid zones;
genotyping by sequencing;
loci;
germplasm;
quantitative traits;
polygenic inheritance;
translation (genetics);
DNA;
databases;
genes;
DNA-binding domains;
cultivars;
abiotic stress;
Medicago sativa;
chromosome mapping
Collection Notes:
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DOI: 10.15482/USDA.ADC/1529208
Citation: Yu, Long-Xi (2018). Data from: Identification of Single-Nucleotide Polymorphic Loci Associated with Biomass Yield under Water Deficit in Alfalfa (Medicago sativa L.) Using Genome-Wide Sequencing and Association Mapping. Frontiers in Plant Science. https://doi.org/10.15482/USDA.ADC/1529208 (Downloaded 2026-06-26)
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