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The ETS domain-containing hematopoietic transcription factor PU.1 mediates the induction of arachidonate 5-lipoxygenase by multi-walled carbon nanotubes in macrophages in vitro

Principal Investigator(s):  View help for Principal Investigator(s) National Institute for Occupational Safety and Health

Version:  View help for Version V1

Name File Type Size Last Modified
1. Quantification of Alox5 mRNA level induced by MWCNTs in macrophages.csv text/csv 859 bytes 06/19/2025 01:59:PM
10. Quantification of Alox5 mRNA level with PU.1 KD.csv text/csv 1.6 KB 06/19/2025 01:59:PM
11. Quantificative measurement of lipid mediators LTB4 and PGE2 with PU.1 KD.csv text/csv 3.2 KB 06/19/2025 01:59:PM
2. Quantification of Alox5 protein level induced by MWCNTs in macrophages.csv text/csv 863 bytes 06/19/2025 01:59:PM
3. Quantificative measurement of LTB4 induced by MWCNTs in macrophages.csv text/csv 1.6 KB 06/19/2025 01:59:PM
4. Quantification of ALOX5 mRNA level induced by MWCNTs in hMDMs.csv text/csv 859 bytes 06/19/2025 01:59:PM
5. Quantificative measurement of LTB4 induced by MWCNT in hMDMs.csv text/csv 846 bytes 06/19/2025 01:59:PM
6. Luciferase activity of mAlox5 5' upstream regulator region.csv text/csv 502 bytes 06/19/2025 01:59:PM
7. Induction of Alox5 promoter activty by MWCNTs.csv text/csv 1.2 KB 06/19/2025 01:59:PM
8. Quantification of PU.1 mRNA level with PU.1 knock-down.csv text/csv 346 bytes 06/19/2025 01:59:PM

Project Citation: 

National Institute for Occupational Safety and Health. The ETS domain-containing hematopoietic transcription factor PU.1 mediates the induction of arachidonate 5-lipoxygenase by multi-walled carbon nanotubes in macrophages in vitro. Ann Arbor, MI: Inter-university Consortium for Political and Social Research [distributor], 2025-06-19. https://doi.org/10.3886/E233583V1

Project Description

Project Title:  View help for Project Title The ETS domain-containing hematopoietic transcription factor PU.1 mediates the induction of arachidonate 5-lipoxygenase by multi-walled carbon nanotubes in macrophages in vitro
Summary:  View help for Summary Fibrogenic multi-walled carbon nanotubes (MWCNTs) polarize macrophages to M1 cells with elevated production of proinflammatory lipid mediators (LMs) to boost acute inflammation. Here we examined the mechanism by which MWCNTs (Mitsui-7) induce arachidonate 5-lypoxygenase (Alox5) critical for production of chemotactic LM leukotriene B4 (LTB4) in murine and human macrophages.

Scope of Project

Subject Terms:  View help for Subject Terms NIOSH-rescue


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Published Versions

V2 [2025-06-23]

V1 [2025-06-19]

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